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Epoch 2 Microplate Spectrophotometer is a compact, monochromator-based microplate spectrophotometer. We use this instrument for microbial growth measurement, micro-volume analysis of DNA/RNA (with TAKE3 plate), and kinetic assays. High-performance liquid chromatography (HPLC), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. In our laboratory, two HPLC systems are available including (1) Agilent 1200 series HPLC equipped with Diode Array Detector (DAD), Fluorescence Detector (FLD), and Automated Fraction Collector (AFC) and (2) Shimadzu Prominence equipped with Multiple Wavelength Detector (MWD) and Refractive Index Detector (RID). Gas chromatography (GC) is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition. In our laboratory, two GC systems are available including (1) Agilent 7890 GC equipped with Flame Ionization detector (FID), Elctron Capture Detector (ECD), liquid autosampler and 7697A headspace autosampler, and (2) Shimadzu GC 2010 Plus equipped with Thermal Conductivity Detector (TCD). Ion chromatography (or ion-exchange chromatography) is a chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger. In our laboratory, Dionex ICS 5000+ Dual Channel equipped with AS-AP Auto Sampler is available, with each channel dedicated to anion and cation.
Shimazdu TOC-L analyzer measures Total Organic Carbon (TOC). The TOC-L series of TOC analyzers adopts the 680°C combustion catalytic oxidation method. Two New Brunswick Scientific BioFlow/CelliGen 115 are available in our laboratory. BioFlow 115 is the most capable and versatile entry-level fermentor/bioreactor ideally suited for a wide range of fermentation and cell culture labs. It features a totally integrated control station with a color touchscreen interface built-in pumps gas flow controllers pH/DO foam/level controllers and more — no external PC needed.
Applied Biosystems MiniAmp PCR Thermal Cycler, Thermo Scientific DNA 120 SpeedVac™ Concentrator, and other equipments are available for basic molecular biology analysis. BioRad CFX Opus 96 Real-Time PCR System was purchased in 2021. CFX Opus is capable of 5 color multiplexing. Coy Vinyl Anaerobic Chamber. The Coy glove bag is a flexible, inflatable PVC chamber with built-in gloves that lets you work in a totally isolated and controlled environment. The chamber provides a strict anaerobic atmosphere using a palladium catalyst and hydrogen gas mix of 5%. Fisherbrand™ Bead Mill 24 Homogenizer. Used for variety of applications that require grinding, lysing, and homogenization of biological samples prior to molecular extraction. Thermo Scientific™ TSX Series Ultra-Low Freezer is used to keep biological samples (DNA, RNA, and bacterial glycerol stocks etc) at -80C. Getinge LSS130 sterilizer. The Getinge Lancer LSS autoclaves are preprogrammed with standard gravity, vacuum, and liquid cycles. LSS 130 configuration features a 4,6 cu.ft. (130 L) chamber with a single door and two extendable shelves. Unisense O2 MicroOptode. A needle type O2 sensor that can be used to pierce through a rubber septum and monitor DO. |